Five-step biotransformation of 1,2,3-trichloropropane into glycerol by the enzymes of the synthetic biodegradation pathway

Five-step biotransformation of 1,2,3-trichloropropane into glycerol by the enzymes of the synthetic biodegradation pathway. The pathway consists of haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 [] with the haloalcohol dehalogenase HheC and epoxide hydrolase EchA from Agrobacterium radiobacter AD1 [, ]. Computer-assisted protein engineering was used to improve activity of the haloalkane dehalogenase towards 1,2,3-trichloropropane, leading to the development of the 32-fold more active and 26-fold more efficient mutant DhaA31 []. Formed glycerol can be utilized in central catabolic pathways of the host cell

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Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway. (2015) Pavel Dvorak, et al. Microb Cell Fact. 2015;14:201. Figure: F1. All organisms Drosophila melanogaster Homo sapiens

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