Biosynthesis pathways of isoprenoids and the biosensors that are responsive to isoprenoids and their precursors

The biosynthesis pathways of isoprenoids and the biosensors that are responsive to isoprenoids and their precursors. (a) The mevalonate (MVA)-responsive biosensors. The MVA auxotroph strain survived only when the MVA was supplied. Otherwise, the absence of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) will cause growth defects. The AraC variant AraC-mev was able to inhibit the corresponding promoter and can be depressed by MVA. (b) The IPP-responsive biosensor. The engineered AraC was coupled with Idi enzyme and can activate the transcription of the corresponding promoter. The activation process was inhibited by IPP. The downstream MutD5 is a mutator; when the mutations which enhance IPP supply were generated, the expression of the mutator will be inhibited. (c) The farnesyl diphosphate (FPP)-responsive biosensors. PgadE was identified to be inhibited by FPP, while PyrbL and PrstA were identified to be activated by FPP. These biosensors have been applied to regulate the MVA pathways and screen for high producers. (d) The geranylgeranyl diphosphate (GGPP)-responsive biosensor. crtR is a repressor to the corresponding promoter and can be released with the binding of GGPP. This biosensor has the potential to regulate downstream carotenoid biosynthesis. (e) The Isoprene-responsive biosensor. TubT activates the transcription of the corresponding promoter with the binding of isoprene. (f) Bicyclic monoterpenes-responsive biosensor. The transcriptional repressor CamR was engineered to respond to some bicyclic monoterpenes. G-3-P, glycerol-3-phosphate; PYR, pyruvate

Publication

Application of Metabolite-Responsive Biosensors for Plant Natural Products Biosynthesis (2023) Jianli Zhang, et al. Biosensors (Basel). 2023 Jun;13(6). Figure: F2. Homo sapiens

Disease mentions