Structural hotspots and signaling pathways that are essential to the hepatic phenotype of highly differentiated HepaRG cells

Structural hotspots and signaling pathways that are essential to the hepatic phenotype of highly differentiated HepaRG cells. (A) Main structural proteins identified in HepaRG cells cultivated in the defined serum-free medium (0.5% DMSO and growth factor supplementation). See also for a comparison with HepG2 cells and PHH, and for a zoom on the zonation factors. (B) Relative differences of protein abundances in HepaRG (defined serum-free medium) and HepG2 cells versus PHH, shown as heatmaps for the β-catenin signaling pathway (white boxes: proteins not seen). Non-detected proteins are colored in grey. (C) Mitochondrial membrane potential (MPT) assessed by JC-1 staining in HepaRG cells cultivated in the differentiation medium (1.7% DMSO, 10% FCS), and treated or not, with or without treatment with the FCCP uncoupler agent; red and green fluorescence indicate hyperpolarization and depolarization, respectively.

Publication

In-Depth Proteome Analysis Highlights HepaRG Cells as a Versatile Cell System Surrogate for Primary Human Hepatocytes. (2019) Georg Tascher, et al. Cells. 2019 Feb;8(2):192. Figure: F3. All organisms Mus musculus Bos taurus Equus asinus Homo sapiens Oryctolagus cuniculus Danio rerio

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