Model pathway for genistein-induced ASAH1 transcription in MCF-7 cells

Model pathway for genistein-induced ASAH1 transcription in MCF-7 cells. Genistein (GEN) activates GPR30, which signals through Gαi to activate c-Src and subsequently the MAPK pathway. ERK1/2 activation promotes the phosphorylation of ERα and its recruitment to the ASAH1 promoter. Induction of ASAH1 gene transcription leads to an increase in protein expression and subsequent up-regulation of enzymatic activity, which results in the degradation of ceramide (CER) into sphingosine (SPH) and its phosphorylation into S1P. S1P is exported from cells via ABC transporters ABCC1 and ABCG2 and acts in a paracrine/autocrine matter to activate proliferative signaling cascades by binding to cell-surface S1P receptors (S1PR). Concomitantly, ASAH1 mediates cyclin B2 expression.
Model pathway for genistein-induced ASAH1 transcription in MCF-7 cells
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Publication

Genistein Stimulates MCF-7 Breast Cancer Cell Growth by Inducing Acid Ceramidase (ASAH1) Gene Expression. (2011) Natasha C. Lucki, et al. J Biol Chem. 2011 Jun 3;286(22):19399-19409. Figure: F7.

Gene mentions


Organism Group Word Match Source NCBI Symbol NCBI ID
Drosophila melanogaster Invertebrates S1P S1P ncbigene_symbol S1P 40399
Drosophila melanogaster Invertebrates (GEN GEN ncbigene_symbol Gen 38594
Drosophila melanogaster Invertebrates ABCC1 ABCC1 ncbigene_synonym MRP 34686
Drosophila melanogaster Invertebrates Ras RAS ncbigene_symbol ras 43873
Drosophila melanogaster Invertebrates Ras RAS ncbigene_synonym Ras64B 38494
Drosophila melanogaster Invertebrates Ras RAS ncbigene_synonym Ras85D 41140
Drosophila melanogaster Invertebrates c-Src C-SRC ncbigene_synonym Src64B 48973
Drosophila melanogaster Invertebrates Raf-1 RAF-1 ncbigene_synonym Raf 31221
Drosophila melanogaster Invertebrates MEK MEK ncbigene_synonym Dsor1 31872
Drosophila melanogaster Invertebrates MEK MEK ncbigene_synonym Mtk 36708
Drosophila melanogaster Invertebrates ERK1 ERK1 ncbigene_synonym rl 3354888
Drosophila melanogaster Invertebrates SK SK ncbigene_symbol SK 31456
Drosophila melanogaster Invertebrates CER CER ncbigene_symbol cer 37229
Drosophila melanogaster Invertebrates SPH SPH ncbigene_symbol sph 44867
Drosophila melanogaster Invertebrates SPH SPH ncbigene_synonym CG5390 34383
Drosophila melanogaster Invertebrates SPH SPH ncbigene_synonym slow-phase 47732
Drosophila melanogaster Invertebrates B2 B2 ncbigene_synonym Prosbeta2 39628
Drosophila melanogaster Invertebrates B2 B2 ncbigene_synonym betaTub85D 41124
Drosophila melanogaster Invertebrates B2 B2 ncbigene_synonym nAChRbeta2 42920
Drosophila melanogaster Invertebrates Sp1 SP1 ncbigene_symbol Sp1 31913
Drosophila melanogaster Invertebrates Sp1 SP1 ncbigene_synonym grass 43273
Drosophila melanogaster Invertebrates Sp1 SP1 ncbigene_synonym Spn42Dd 49808
Drosophila melanogaster Invertebrates ERa) ERA ncbigene_symbol era 5658294
Homo sapiens Primates S1P S1P ncbigene_synonym MBTPS1 8720
Homo sapiens Primates (GEN GEN ncbigene_synonym GEN1 348654
Homo sapiens Primates ABCC1 ABCC1 ncbigene_symbol ABCC1 4363
Homo sapiens Primates ABCG2 ABCG2 ncbigene_symbol ABCG2 9429
Homo sapiens Primates GPR30 GPR30 ncbigene_synonym GPER1 2852
Homo sapiens Primates S1PR S1PR famplex_relations S1PR1 1901
Homo sapiens Primates S1PR S1PR famplex_relations S1PR2 9294
Homo sapiens Primates S1PR S1PR famplex_relations S1PR3 1903
Homo sapiens Primates S1PR S1PR famplex_relations S1PR4 8698
Homo sapiens Primates S1PR S1PR famplex_relations S1PR5 53637
Homo sapiens Primates Ras RAS famplex_relations KRAS 3845
Homo sapiens Primates Ras RAS famplex_relations HRAS 3265
Homo sapiens Primates Ras RAS famplex_relations NRAS 4893
Homo sapiens Primates c-Src C-SRC ncbigene_synonym SRC 6714
Homo sapiens Primates Gj GJ famplex_relations GJA1 2697
Homo sapiens Primates Gj GJ famplex_relations GJA10 84694
Homo sapiens Primates Gj GJ famplex_relations GJA3 2700
Homo sapiens Primates Gj GJ famplex_relations GJA4 2701
Homo sapiens Primates Gj GJ famplex_relations GJA5 2702
Homo sapiens Primates Gj GJ famplex_relations GJA8 2703
Homo sapiens Primates Gj GJ famplex_relations GJA9 81025
Homo sapiens Primates Gj GJ famplex_relations GJB1 2705
Homo sapiens Primates Gj GJ famplex_relations GJB2 2706
Homo sapiens Primates Gj GJ famplex_relations GJB3 2707
Homo sapiens Primates Gj GJ famplex_relations GJB4 127534
Homo sapiens Primates Gj GJ famplex_relations GJB5 2709
Homo sapiens Primates Gj GJ famplex_relations GJB6 10804
Homo sapiens Primates Gj GJ famplex_relations GJB7 375519
Homo sapiens Primates Gj GJ famplex_relations GJC1 10052
Homo sapiens Primates Gj GJ famplex_relations GJC2 57165
Homo sapiens Primates Gj GJ famplex_relations GJC3 349149
Homo sapiens Primates Gj GJ famplex_relations GJD2 57369
Homo sapiens Primates Gj GJ famplex_relations GJD3 125111
Homo sapiens Primates Gj GJ famplex_relations GJD4 219770
Homo sapiens Primates Gj GJ famplex_relations GJE1 100126572
Homo sapiens Primates Raf-1 RAF-1 ncbigene_synonym RAF1 5894
Homo sapiens Primates MEK MEK ncbigene_synonym MAP2K7 5609
Homo sapiens Primates MEK MEK famplex_relations MAP2K1 5604
Homo sapiens Primates MEK MEK famplex_relations MAP2K2 5605
Homo sapiens Primates ERK1 ERK1 ncbigene_synonym MAPK3 5595
Homo sapiens Primates ERK1/2 ERK2 ncbigene_synonym MAPK1 5594
Homo sapiens Primates SPH SPH ncbigene_synonym SFTA3 253970
Homo sapiens Primates ASAH1 ASAH1 ncbigene_symbol ASAH1 427
Homo sapiens Primates >cyclinB2 CCNB2 ncbigene_symbol CCNB2 9133
Homo sapiens Primates B2 B2 ncbigene_synonym NEUROD1 4760
Homo sapiens Primates B2 B2 ncbigene_synonym TUBB4B 10383
Homo sapiens Primates Sp1 SP1 ncbigene_symbol SP1 6667
Homo sapiens Primates Sp1 SP1 ncbigene_synonym PSG1 5669
Homo sapiens Primates Sp1 SP1 ncbigene_synonym DAND5 199699
Homo sapiens Primates ERa) ERA ncbigene_synonym ESR1 2099
Homo sapiens Primates ERa) ERA ncbigene_synonym ERAL1 26284

Chemical mentions

Word Match MeSH Name ChEBI

Disease mentions

Word Match MeSH Name DOID
Cancer NA