KLA increases mRNA expression of genes involved in sphingolipid biosynthesis

KLA increases mRNA expression of genes involved in sphingolipid biosynthesis. Gene expression data for the sphingolipid metabolic enzymes following 24 h of treatment with KLA (100 ng/ml) using the KEGG-based microarray analysis tool (GenMAPP version 2.1) that has been modified to display this pathway more completely. The coloration is log2-fold change in expression following treatment with KLA (100 ng/ml) for 24 h from microarray data generated by the LIPID MAPS Consortium. The sphingolipid metabolites and genes (with the gene abbreviations shown in boxes or enzyme names where gene names are ambiguous) are given for the condensation of serine and palmitoyl-CoA to form 3-ketosphinganine (3-ketoSa) by serine palmitoyltransferase (SPT), which is reduced to sphinganine (Sa), acylated to dihydroceramides (DHCer) by (DH)Cer synthases (CerS), and incorporated into more complex DH-sphingolipids (the 1-phosphate (DHCer-1-P) sphingomyelins (DHSM), glucosylceramides (DHGlcCer), galactosylceramides (DHGalCer)) or desaturated to Cer followed by headgroup addition. Also included are a number of the catabolic genes, e.g. sphingomyelinases (SMPD), ceramidases (ASAH), sphingosine kinases (SphK) for the formation of sphinganine 1-phosphate (Sa1P) and sphingosine 1-phosphate (So1P), and phosphatases for the reverse reaction and the lyase that cleaves sphingoid base 1-phosphates to ethanolamine phosphate (EP), hexadecanal (C16:0al), and hexadecenal (C16:1al).

Publication

Kdo2-Lipid A, a TLR4-specific Agonist, Induces de Novo Sphingolipid Biosynthesis in RAW264.7 Macrophages, Which Is Essential for Induction of Autophagy. (2010) Kacee Sims, et al. J Biol Chem. 2010 Dec 3;285(49):38568-38579. Figure: F5. All organisms Homo sapiens Mus musculus Bos taurus Oryctolagus cuniculus Capra hircus Drosophila melanogaster

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