A working model for endosomal transport involving CLC-5 and KIF3B interaction in polarized cells

Schematic representation of a working model for endosomal transport involving CLC-5 and KIF3B interaction in polarized cells. CLC-5 and KIF3B are expressed in polarized renal proximal tubular cells which reabsorb proteins and solutes via the receptor-mediated endocytic pathway that uses megalin and cubilin as the apical plasma membrane (APM) receptors (, , ). Ligands bind to these receptors at the APM where CLC-5 and interacting proteins Nedd4-2, NHERF2, and cofilin are also present (, , ). The receptor-ligand complex is internalized in vesicles, containing CLC-5 and CLC-4 (), which are anterogradely transported along microtubules via the Kinesin-2 complex (). These vesicles fuse with early endosomes where acidification involves the vacuolar H+-ATPase, with counter ions being provided by CLC-5 (). In nonpolarized cells, the orientation of the microtubules is reversed (), and CLC-5 vesicles would be transported to the cell surface.

Publication

CLC-5 and KIF3B interact to facilitate CLC-5 plasma membrane expression, endocytosis, and microtubular transport: relevance to pathophysiology of Dent's disease. (2010) Anita A. C. Reed, et al. Am J Physiol Renal Physiol. 2010 Feb;298(2):F365-F380. Figure: F7. All organisms Mus musculus Homo sapiens Rattus norvegicus Canis lupus familiaris Oryctolagus cuniculus Bos taurus Capra hircus Drosophila melanogaster

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