CLR-based networks of genes whose expression changed significantly due to aminoglycoside (gentamicin or kanamycin) treatment

CLR-based networks of genes whose expression changed significantly due to aminoglycoside (gentamicin or kanamycin) treatment. In this figure, upregulated gene expression is shown in green, downregulated gene expression is in yellow, and non-perturbed gene expression is shown in light blue. Overlaid on these networks are the outliers from a screen of the single-deletion knockout (KO) library grown in the presence of 5µg/ml gentamicin (increased growth of KO, red rim; decreased growth of KO, blue rim; no change in growth of KO, black rim). We note that no single-knockouts of genes in these networks show decreased growth in the presence of gentamicin. (A) Network of genes showing pathway enrichment for the electron transport chain (ETC), tricarboxylic acid (TCA) cycle and respiration (p < 10−11), and transcription factor enrichment for metabolic regulators including ArcA (p = 1.8×10−12). (B) Network of genes showing pathway enrichment for the response to misfolded proteins (p = 1×10−17) and the heat shock sigma factor, σH (RpoH: p = 1.2×10−11).

Publication

Mistranslation of membrane proteins and two-component system activation trigger aminoglycoside-mediated oxidative stress and cell death. () Michael A. Kohanski, et al. Cell. ;135(4):679-690. Figure: F2. Escherichia coli str. K-12 substr. MG1655

Disease mentions