Model for repair of camptothecin-induced replicative damage

Model for repair of camptothecin-induced replicative damage. The drawing depicts selected DNA lesions and repair intermediates (numbered) that might occur after a replisome (large oval) collides with a Top1 cleavage complex (small oval). The outcomes vary depending on whether Top1 was attached to the leading strand template (Top) or lagging strand template (Bottom). Possible repair steps are indicated by arrows, including enzymatic reversal of the Top1 cleavage complex by Top1 itself, repair that includes Top1 removal by Tdp1, and repair that includes Top1 removal by Rad1-Rad10. Smaller pathway arrows indicate less favored possibilities. Arrowheads in the drawings indicate DNA synthesis by a replication fork. The dashed line represents repair synthesis that must occur before formation of the recombination D-loop. See text for further discussion.

Publication

Yeast Tdp1 and Rad1-Rad10 function as redundant pathways for repairing Top1 replicative damage. (2002) John R. Vance, et al. Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13669-13674. Figure: F5. All organisms Saccharomyces cerevisiae S288C Homo sapiens

Disease mentions