Components of the AhR-mediated lacZ reporter assay

Components of the AhR-mediated lacZ reporter assay. The key elements of the β-galactosidase (lacZ) reporter plasmids (pLXRE5-Z and pTXRE5-Z) and the AHR-GAL 1,10 promoter-ARNT expression construct in chromosome III are shown. The reporter plasmids are centromeric (low copy number) and contain either leucine or tryptophan biosynthetic pathway genes as selectable markers, respectively. AhR/Arnt-mediated expression of the lacZ reporter gene is driven by 5 upstream AHREs. AhR, aryl hydrocarbon receptor; Arnt, aryl hydrocarbon nuclear translocator; AHRE, aryl hydrocarbon response elements
Components of the AhR-mediated lacZ reporter assay
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Publication

Cooperation of heat shock protein 90 and p23 in aryl hydrocarbon receptor signaling. (2004) Marc B. Cox, et al. Cell Stress Chaperones. 2004 Mar;9(1):4-20. Figure: F1.

Gene mentions


Organism Group Word Match Source NCBI Symbol NCBI ID
Homo sapiens Primates lac LAC ncbigene_synonym LCT 3938
Homo sapiens Primates AHR AHR ncbigene_symbol AHR 196
Homo sapiens Primates ARNT ARNT ncbigene_symbol ARNT 405
Homo sapiens Primates ARNT ARNT ncbigene_synonym MIA3 375056
Homo sapiens Primates GAL GAL ncbigene_symbol GAL 51083
Homo sapiens Primates GAL GAL ncbigene_synonym GALP 85569
Drosophila melanogaster Invertebrates lac LAC ncbigene_symbol Lac 36363
Drosophila melanogaster Invertebrates lac LAC ncbigene_synonym lacquered 44521
Drosophila melanogaster Invertebrates AHR AHR ncbigene_synonym ss 41988
Drosophila melanogaster Invertebrates ARNT ARNT ncbigene_synonym tgo 41084
Drosophila melanogaster Invertebrates GAL GAL ncbigene_symbol Gal 33839
Drosophila melanogaster Invertebrates Chr. CHR ncbigene_symbol chr 250498
Drosophila melanogaster Invertebrates Chr. CHR ncbigene_synonym Chro 40508
Drosophila melanogaster Invertebrates Chr. CHR ncbigene_synonym RabX1 44172
Escherichia coli str. K-12 substr. MG1655 Bacteria lacZ LACZ ncbigene_symbol lacZ 945006
Escherichia coli str. K-12 substr. MG1655 Bacteria AHR AHR ncbigene_symbol ahr 948802
Escherichia coli str. K-12 substr. MG1655 Bacteria ARNT ARNT ncbigene_symbol arnT 947297

Chemical mentions

Word Match MeSH Name ChEBI

Disease mentions

Word Match MeSH Name DOID