The main findings on HMEC-1

Summary of the main findings on HMEC-1. GlcN is incorporated into the cell by glucose transporters (purple line), then is incorporated into the hexosamine biosynthetic pathway (HBP), where the uridine diphosphate (UDP)-GlcNAc substrate is used by the OGT enzyme for O-GlcNAc (blue box) several proteins, while the enzyme OGA removes it. GlcN treatment increases O-GlcNAcylation (blue lines), reactive oxygen species (ROS) production, and nuclear factor erythroid-2-related factor 2 (Nrf2) expression in human dermal microvascular endothelial cells-1 (HMEC-1). The inhibition of O-GlcNAcylation, with OSMI-1, prevents (orange lines) ROS production and Nrf2 expression, suggesting that these processes are related. In addition, GlcN also could scavenge some ROS. Biorender.com GPx = glutathione peroxidase, CAT = catalase, SOD = superoxide dismutase, GSH = glutathione, NQO1 = NAD(P)H quinone oxidoreductase 1, HOCl = hypochlorous acid, ONOO– = peroxynitrite anion, O2•- = superoxide anion, ROO• = peroxyl radicals, H2O2 = hydrogen peroxide, OH• = hydroxyl radicals, HMEC-1 = human dermal microvascular endothelial cells-1. Created with BioRender.com.

Publication

Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells (2023) B. Fernández-Rojas, et al. Curr Res Toxicol. 2023;5(2):100128. Figure: F7. Homo sapiens

Disease mentions